Considering the fact that fast-growing strains with high biomass productivity and lipid content are the most important requirements for the provision of bioenergy generation from cyanobacteria, the results presented here confirm that lipid extracted from Synechococcus sp. can be used as a promising feedstock for biodiesel production. Ostrich oil has been valued for its numerous benefits to human health for a long time. To be able to reduce production costs, whilst increasing lipid extraction yield, ostrich oil, as a low-priced byproduct of the ostrich industry, might be a promising alternative substance for the production of clean fuels. We are continuously striving to isolate efficient lipid-producing cyanobacterial strains by using cheaper alternative byproducts, with our main aim being to improve biofuel production yield.