Differential scanning calorimetric studies were performed in
a Polymer Laboratories calorimeter (Rheometric Scientific Ltd.)
with a Plus V 5.41 software. The equipment was calibrated at
a heating rate of 10 C/min using indium as standard. Samples
(18–22 mg wet weight) were placed in DSC hermetic pans and
scanned from 15 to 100 C at a b ¼10 C/min by triplicate. As
reference, a capsule with distilled water was used. Denaturation
enthalpies (DHmyosin, DHactin and DHtotal) were estimated by
measuring the corresponding areas under the DSC transition
curve. Specific areas (partial area/total mg of dry sample) were
calculated.