PCR reactions were performed in a volume of 60 mL containing 2 mL lysate as template, 1 unit of BIOTAQ™DNA polymerase (DNA-Technology), 1 mM dNTP, 1.5 mM MgCl2, and 1 mM of the primers.
PCR reactions were performed in a volume of 60 mL containing2 mL lysate as template, 1 unit of BIOTAQ™DNA polymerase(DNA-Technology), 1 mM dNTP, 1.5 mM MgCl2, and 1 mM of theprimers.