There was significant difference in the carbohydrate contents between the fermented and unfermented cotton seeds. However, a noticeable increase in the carbohydrate content was observed in the fermented cotton seeds. This increase in value ranged from 6.44% to 15.72% in the unfermented cotton seeds and fermented cotton seeds using a combination of all the bacterial cultures in inoculating, respectively. This could be because the microorganisms did not hydrolyse carbohydrate in their fermentative activity.