After the incubation time, larvae and juveniles were separated from the test media,
briefly dried on filter paper, quickly immersed in mineral oil to avoid evaporation and
desiccation, and the remaining adherent water was aspirated through a glass micropipette.
Subsequently, the hemolymph was sampled with a new micropipette inserted
in the heart. In adult crabs, the hemolymph was collected via a hypodermic needle after
section of the propodite of a fourth or fifth pereiopod previously dried with filter paper.
The hemolymph was quickly transferred into mineral oil to avoid evaporation