Zygosaccharomyces rouxii V19 was grown in
YPG medium (yeast extract, 0.5%, peptone, 1.0%, glucose,
10%). Fermented broth was purified through a series
of ion-exchange columns and ODS column and the purified
sample was TMS-esterified. Malic and succinic acids were
identified with GC-MS analysis. The yeast was cultivated
under various cultural conditions and quantitative determination
of the organic acids was carried out with HPLC
on Shodex column. Glucose concentration of 30%, initial
pH 5.0, and 25 ◦C incubation temperature were the optimum
conditions. Inclusion of glutamic, malic, and succinic
acid precursors in the medium increased the production
of malic acid. On the other hand, only addition of malic
acid enhanced the production of succinic acid. Maximum
amount of malic acid produced was 74.9 g/L (32.8% yield,
based on glucose consumption) in the medium with 0.5%
glutamic acid supplement, and that of succinic acid was
7.7 g/L (8.1% yield) when 0.3% malic acid was added in
the medium.
Zygosaccharomyces rouxii V19 was grown inYPG medium (yeast extract, 0.5%, peptone, 1.0%, glucose,10%). Fermented broth was purified through a seriesof ion-exchange columns and ODS column and the purifiedsample was TMS-esterified. Malic and succinic acids wereidentified with GC-MS analysis. The yeast was cultivatedunder various cultural conditions and quantitative determinationof the organic acids was carried out with HPLCon Shodex column. Glucose concentration of 30%, initialpH 5.0, and 25 ◦C incubation temperature were the optimumconditions. Inclusion of glutamic, malic, and succinicacid precursors in the medium increased the productionof malic acid. On the other hand, only addition of malicacid enhanced the production of succinic acid. Maximumamount of malic acid produced was 74.9 g/L (32.8% yield,based on glucose consumption) in the medium with 0.5%glutamic acid supplement, and that of succinic acid was7.7 g/L (8.1% yield) when 0.3% malic acid was added inthe medium.
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