Most analytical methods for the determination of enzyme activity
may be divided into enzymatic, which is the focus of this research,
and immunological, according to the mechanism involved in the
reaction. The enzymatic methods are based on the hydrolysis
undergone by a specific substrate in contact with the enzyme
which releases a product of low molecular weight formed during
the reaction, proportional to the bioactive enzyme and able to be
quantified using colorimetric or fluorimetric methods