In order to evaluate the activity of Pa-MAP 2, the MICs where determined against E. coli. Pa-MAP 2 linked to ELP-intein did not show activity at the highest concentration tested (200 M). Purified recombinant Pa-MAP 2 and chemically synthesized Pa-MAP 2,both with purity higher than 95%, inhibited E. coli growth at theconcentration between 12 and 25 M in MIC analysis.