Conditions of experiment (in each glass jar)
temperature 26 ±1°C
Dissolved Oxygen (DO) > 5 mg/L
photoperiod was held at 14:10 (Light : Dark )
Dilution water (hardness 80 mg/L as CaCO3 equivalents, pH = 8.0±0.5)
treat chlorine every 48 h
fed food pellets (35 % protein, 11.12 % lipids and 20.84 % carbohydrates) amount equivalent to 5 % of body mass
supplemented with Elodea sp. (fresh leaves)