The preferred nitrogen source for
optimum accumulation of lipids was ammonium chloride, besides
a proportion of VFA (acetic, propionic and butyric acids) of 8:1:1,
which demonstrated the yeast preference for acetic acid. This
study also stated that the higher the initial concentration of VFA
the higher the accumulation of lipids. In a two-stage fed-batch fermentation
strategy, Y. lipolytica MUCL 28849 was initially grown
on glucose or glycerol and subsequently VFA were added under
nitrogen limitation and after glucose or glycerol exhaustion.
into methyl esters through transesterification. This would make
necessary the inclusion of lipid pretreatment.