Bio-polishing was carried out in a Launder-O-Meter (LOM) LP2 from Atlas Electric Devices Company. Twenty steel balls, buffer and enzyme were added to 500mL steel beakers. The 50 mM sodium bicarbonate buffer was adjusted to pH 8 and used for all experiments. The liquor ratio was 20:1 and the enzymes were dosed as U/ml (LU or ECU per liquor volume). Two swatches with a total weight of 7.00.1 g were used in each beaker and each treatment was run in duplicate. The beakers were loaded into a LOM preheated to 70°C and
incubated at 42 rpm for a specified time. Following enzyme treatment, the residual enzyme activity on the swatches was inactivated at 80°C in 2 g/L soda ash solution for 10 minutes. After inactivation, the swatches were rinsed and centrifuged in a conventional home washer and tumble dried for 60 minutes. All swatches were conditioned overnight at a constant temperature and humidity (70 2°F, 65 2% RH).