Fig. 4. Reactive oxygen species generation in embryos treated with Ag+ and AgNPs at 72 hpf. The representative images from three different experiments are shown. The left column contains the bright field images, whereas the right column contains the same image using the GFP filter. (A and B) control embryo. (C and D) Embryo exposed to 0.03 lgmL1 Ag+. (E and F) Embryo exposed to 0.31 lg mL1 Ag+. (G and H) Embryo exposed to 0.03 lgmL1 AgNP. (I and J) Embryo exposed to 0.31 lg mL1 AgNP. The percentage on each of the fluorescent images refers to the percentage of embryos displaying the phenotype on the image. (K) ROS-score obtained from assigning the fluorescent images a score of 1 if the image resembled the majority of the controls and 2 if it did not (statistical differences are indicated by an asterisk).