FCIS was collected by tapping the unopened spadix of the palm from three trees of the C. nucifera L.
and were stored in a container which had been thoroughly washed with boiled water, and then desiccated to
avoid microbial contamination. The FCIS was collected and transported to the laboratory maintaining 4°C until
processing. A portion of each sap was quickly filtered (Whatman No.1), put into a conical flask covered with
cheese cloth, and immediately used for tests and then fermented for about 24 hrs to 3 days at 25 ± 2°C, respectively in triplicate. An aliquot of the sap was removed from each conical flask and the physicochemical
compositions were investigated periodically. Sugar samples (1 g) were dissolved in 10 mL of distilled water.