A spring wheat, Triticum aestivum cv. Bobwhite, was used
through out this study. Stock plants were grown in an environmentally controlled growth chamber maintained at 18/16C (day/night) and a 16/8-h (day/night) photoperiod with light provided by high intensity discharge lights at 800 mmol m-2 s-1. Immature caryopses were collected from the plants 12–14 days after anthesis. Immature embryos were dissected aseptically and cultured on a solid CM4C medium with 100 mg/l ascorbic acid for 4 days before
transformation.