In our previous work, Cibacron blue F3GA-immobilized nylon
membrane was prepared and the adsorption and desorption of
papain were investigated [18]. We have extended these studies
using two new nylon affinity membranes that include Red 120
and Brown 10 as possible affinity ligands for papain. The composite
membranes were directly used for papain purification from
papaya powder as apposed fromsolubilized papain extracts as previously
reported [18]. The effects of pH, loading concentration of
the enzyme, ionic strength and temperature on the effectiveness of
adsorptionwere studied using this batchmethod. The performance
of the membrane stack was also evaluated.