the constituents of culture edium play an importtant role in the process of tissue differentiation. the beneficial effect of cytokkinins(BA)has been reported in the induction of shoot bud primordia in different crop plants10, which may be due to more synthesis of nucleic acids and proteins required for organogensis11. the cytokinins are generally added to induce shoot foration and to inhibit root formation. in citrus also, the role of cytokinins in shoot regenration has been well documented12.