3. Results and discussion
3.1. Production of ethanol from hexoses
The fermentation performance of T. hirsuta was investigated
in media containing monosaccharides as carbon sources. The
monosaccharides used were glucose, mannose, fructose and galactose.
Although glucose and mannose were consumed completely
within 72 h, the consumption of fructose and galactose by T. hirsuta
was slower. T. hirsuta was cultured in 20 g/l glucose and mannose,
showing maximum ethanol concentrations of 9.7 and 9.6 g/l after
72 and 96 h cultivation, resulting in ethanol yields of 0.49 and
0.48 g/g sugar utilized, respectively (Fig. 1A and B). In the case of
fructose and galactose,maximumethanol concentrations of 5.4 and
3.3 g/l were observed after 144 h cultivation, resulting in ethanol
yields of 0.46 and 0.45 g/g sugar utilized, respectively (Fig. 1C and
D). Additionally, when the fungus was fermented on a medium
containing 20 g/l cellobiose and maltose as disaccharides, both disaccharides
were decomposed to glucose gradually, and produced
maximum ethanol concentrations of 9.4 and 9.4 g/l after complete
consumption of the sugars. This resulted in ethanol yields of 0.47
and 0.47 g/g cellobiose and maltose, respectively (Fig. 2A and B),
indicating that T. hirsuta can effectively convert cellobiose and maltose
to ethanol. Thus, T. hirsuta is advantageous over S. cerevisiae as
it can ferment disaccharides.
3. Results and discussion3.1. Production of ethanol from hexosesThe fermentation performance of T. hirsuta was investigatedin media containing monosaccharides as carbon sources. Themonosaccharides used were glucose, mannose, fructose and galactose.Although glucose and mannose were consumed completelywithin 72 h, the consumption of fructose and galactose by T. hirsutawas slower. T. hirsuta was cultured in 20 g/l glucose and mannose,showing maximum ethanol concentrations of 9.7 and 9.6 g/l after72 and 96 h cultivation, resulting in ethanol yields of 0.49 and0.48 g/g sugar utilized, respectively (Fig. 1A and B). In the case offructose and galactose,maximumethanol concentrations of 5.4 and3.3 g/l were observed after 144 h cultivation, resulting in ethanolyields of 0.46 and 0.45 g/g sugar utilized, respectively (Fig. 1C andD). Additionally, when the fungus was fermented on a mediumcontaining 20 g/l cellobiose and maltose as disaccharides, both disaccharideswere decomposed to glucose gradually, and producedmaximum ethanol concentrations of 9.4 and 9.4 g/l after completeconsumption of the sugars. This resulted in ethanol yields of 0.47and 0.47 g/g cellobiose and maltose, respectively (Fig. 2A and B),indicating that T. hirsuta can effectively convert cellobiose and maltoseto ethanol. Thus, T. hirsuta is advantageous over S. cerevisiae asit can ferment disaccharides.
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