was used to define the calculated F value and relate it to the tabulated F value for Regression Degree of
Freedom equal to 6 and Residue Degree of Freedom equal to 4. The analysis of the significance of
models shown that the models of times 6 h, 12 h and 54 h are statistically significant, as they have
Fcalc/Ftab > 1 (Table 3). Thus, for these models, response surfaces shown in Figure 1, Figure 2 and
Figure 3, respectively, were made.