negative strainM16. The same strain did not showany significant differences
in the level of PHYPk gene expression in any of the two media
(data not shown). The results obtained from the analysis using 2−ΔΔCt
method (data normalized on LSC2 and ACT1, respectively) matched
with those obtained by copy number analysis (CN) (Supplementary
material, Fig. S1). The trend of the expression of the genewas comparable
and the only variation was the evaluation of the statistical
differences.