Results and discussion
3.1. Microsatellite analysis and polymorphism
The standard method of DNA isolation and PCR parameters evaluation for microsatellite amplification is the first phase of the study which involves the analysis of the four populations used. The marker selection is done by selecting the locus which differentiate the high and low group (Fig. 1). The polymorphic markers in each group of each locus were then tested for each individual in the group. The results from the 50 markers evaluated, there were 28 polymorphic marker used for subsequent analysis. 15 polymorphic marker contained in OB population, 18 marker in BN population, 11 marker in OY population, and 13 marker in FFB population.