Low molecular weight (50–190 kDa) chitosan was purchased from Sigma–Aldrich Ltd., USA. A 2% chitosan stock solution was solubilized by mixing 16 g of chitosan into 800 mL of sterile 1% aqueous acetic acid in a Waring blender for 10–15 min.
Low molecular weight (50–190 kDa) chitosan was purchasedfrom Sigma–Aldrich Ltd., USA. A 2% chitosan stock solution wassolubilized by mixing 16 g of chitosan into 800 mL of sterile 1%aqueous acetic acid in a Waring blender for 10–15 min.