In order to eliminate the possibility of solvents used with both variants of diffusion method to cause sensitivity differences, another experiment was performed employing diffusion method variant disc, using DMSO to dissolve extracts (DMSO was used to test variant well of diffusion method, data shown in Table 4). The results of this experiment, were compared with the values of zones of growth inhibition previously found
by both diffusion method variants (discs loaded with solvents other than DMSO and well filled with DMSO) and indicated that DMSO did not cause significant difference between the two diffusion method variants, [GL(2,24); F = 12,6; p > 0.01]. Despite the limitations of the present study, we can assume that the results suggest that both variants display good precision.