Powdered leaves were extracted by four different systems.
Plant material (10 g) was processed by autoclaving it in 100 mL of water (AW)
or by soaking it, separately, in 100 mL of water (W),
ethanol (EteOH) or ethyl acetate (Et-OAc) for 24 h at room temperature.
Each extract was then filtered through two layers of Whatman