At the beginning of the fermentation, the living spore number was few due to it need to adaptthe new environment (in Fig.4), however, as the cultivate time went by, the bacteria entered into the logarithmic phase, the metabolism of cells became very fast, after 48 h fermentation, it went into stable period, the initial pH value of 7 and 9 got the highest spores 1.03×1011, which was much higher than any other, this conclusion was the same with the δendotoxin.