Keratins extracted by sulphitolysis were characterized by pro-teins having molecular weights of about 60 and 45 kDa (low sulphur
content keratins) and proteins having molecular weights ranging
from 28 and 11 kDa (high sulphur content keratins) [19]; the iso-electric point of the keratins fell in the range between 4 and 4.5
[20]. Moreover, keratins contained several amino acids with polar
side residues able to bind cationic substances: amino acids as aspar-tic acid, glutamic acid, arginine, cystine and cystein-S-sulphnoate
residues composed more than 38% of total amino acid content [21].
The keratin nanofibrous membrane was robust, free-standing
and flexible; therefore it could be formed into various membrane
modules (Fig. 1a). The membrane was made of randomly oriented
nanofibres having a mean diameter of about 220 nm (Fig. 1b). The
nanofibres did not show defects as beads or flat morphologies
(Fig. 1b); however the diameter distribution curve was rather broad
extending from 70 to 430 nm (as shown in Fig. 1c).
As regards the keratin nanofibrous membrane properties, the
specific surface area was of 13.59 m2
g
−1
and the porosity was of
about 90% (Table 1).
Keratins extracted by sulphitolysis were characterized by pro-teins having molecular weights of about 60 and 45 kDa (low sulphur
content keratins) and proteins having molecular weights ranging
from 28 and 11 kDa (high sulphur content keratins) [19]; the iso-electric point of the keratins fell in the range between 4 and 4.5
[20]. Moreover, keratins contained several amino acids with polar
side residues able to bind cationic substances: amino acids as aspar-tic acid, glutamic acid, arginine, cystine and cystein-S-sulphnoate
residues composed more than 38% of total amino acid content [21].
The keratin nanofibrous membrane was robust, free-standing
and flexible; therefore it could be formed into various membrane
modules (Fig. 1a). The membrane was made of randomly oriented
nanofibres having a mean diameter of about 220 nm (Fig. 1b). The
nanofibres did not show defects as beads or flat morphologies
(Fig. 1b); however the diameter distribution curve was rather broad
extending from 70 to 430 nm (as shown in Fig. 1c).
As regards the keratin nanofibrous membrane properties, the
specific surface area was of 13.59 m2
g
−1
and the porosity was of
about 90% (Table 1).
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