The in vitro flowering is useful for shortening the period of breeding program due to the
shorter time of flowering compare to natural. Moreover, the in vitro flowering of ornamental plants
can be sale as a souvenir at higher price, thus, it is a product’s value added. This research
aimed to find the culture medium that can induce in vitro flowering of dwarf Oncidium ‘JairakRainbow’ orchid. The plantlets were cultured on modified MS (1962) medium by lower nitrogen
content in the form of NH4NO3 to 20 times and/or increase phosphorous content in the form of
KH2PO4 to 5 times. Plant growth regulator BA at the concentration of 10 mg/l was also tested. The
results demonstrated that BA could induce multiple shoots, but shoots were small and no new
root emerged. The media contained 5xP and 1/20xN plus 10 mg/l BA yielded highest shoots
number. The increase of P and decrease of N without BA addition did not show significant on
growth. For the medium in vitro flowering, MS+10 mg/l BA and MS with 5xP plus 10 mg/l BA,
were only two medium that induced in vitro flowering. However, the flowering induction was very
low as only 14.3%
The in vitro flowering is useful for shortening the period of breeding program due to theshorter time of flowering compare to natural. Moreover, the in vitro flowering of ornamental plantscan be sale as a souvenir at higher price, thus, it is a product’s value added. This researchaimed to find the culture medium that can induce in vitro flowering of dwarf Oncidium ‘JairakRainbow’ orchid. The plantlets were cultured on modified MS (1962) medium by lower nitrogencontent in the form of NH4NO3 to 20 times and/or increase phosphorous content in the form ofKH2PO4 to 5 times. Plant growth regulator BA at the concentration of 10 mg/l was also tested. Theresults demonstrated that BA could induce multiple shoots, but shoots were small and no newroot emerged. The media contained 5xP and 1/20xN plus 10 mg/l BA yielded highest shootsnumber. The increase of P and decrease of N without BA addition did not show significant ongrowth. For the medium in vitro flowering, MS+10 mg/l BA and MS with 5xP plus 10 mg/l BA,were only two medium that induced in vitro flowering. However, the flowering induction was verylow as only 14.3%
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