To the best of the authors’ knowledge, there are some meth-ods investigating PFAS concentrations in dairy and human milk[5,15,18–21]; yet detailed characterization and descriptions ofmethod parameters was limited to two of them [5,15].This study describes a new and easy LC/ESI–MS/MS methodfor the determination of 12 perfluorinated acids in milk. In par-ticular we developed an enrichment method using graphitizedcarbon black (GCB) which may allow the selective isolation of ana-lytes target from complexes matrices thanks to the various typesof interactions (hydrophobic, electronic and ion-exchange) withanalytes. GCB sorbents have been applied in various enrichmenttechniques, particularly in SPE