2.8.2. Sugarcane juice Mixed juice (12.7 8Brix; pH 6.1; 3177 ppm haze dextran/
8Brix) was obtained from a Louisiana factory and stored in a 80 8C freezer until used. Dextranases 2–4werefirst diluted (0.03 g/100 ml) in pH 5.4 acetate buffer. The ‘‘concentrated’’ enzyme 2 had also been previously diluted 4.6X to make it economically equivalent to the nearest priced ‘‘non-concentrated’’ dextranase. Diluted enzyme (1 ml) was added to the juice (2 ml) in a test-tube, coverered with aluminum foil, and thoroughly mixed. For the control, de-ionized water (1 ml) was added instead of dilute enzyme. The test-tubes were placed in a shaking water-bath (90 rpm) at different temperatures ranging from 26.6 to 65.5 8C for 25 min. After incubationthetest-tubeswereimmediatelyplacedinaboiling water-bath for 2.5 min, and cooled on ice. Duplicate aliquots were diluted (0.75 ml/50 ml de-ionized water) and filtered through a 0.45 mm membrane, before IC–IPAD analyses.