In the present study callus formed from leaf segments cultured in a combination of BA with NAA and BA with Kinetin. No shoot morphogenesis occurred from explants cultured on any test combinations of auxins and cytokinins (data not shown). A number of well developed plantlets of root and shoot systems were successfully hardened and acclimatized using a grade mixture consisting of peatmoss+ loam+ styrofoam (1: 1: 0.5). The hardened plants (Fig. 5, J and K) were established in soil during (3) weeks.