Standard
assay conditions (1.0 mL final volume) were 50 mmol L−1 Hepes
buffer, pH 7.5, 0.28 mmol L−1 NADH, 4.2 mmol L−1 PEP, PK (60 U),
LDH (230 U), 50 μmol L−1 orthovanadate, 1 μg alamethicin/μg protein
and 3 mmol L−1 KCl (to ensure adequate PK activity).