The blg/Lyso microspheres may be produced by monitoring the
couple pH/isoelectric point (pI), which in turn promotes electrostatic
interactions between the proteins (Diarrassouba et al.,
2013). At the optimal pH for the interaction (pH 7.5), blg is negatively
charged while Lyso carries a net positive charge. The pI of
blg (5.2) is below pH 7.5 and that of Lyso (11.5) is above pH 7.5