2.4. Yeast phase product inoculation
Yeast-like growth was induced by inoculation of hypha/spore suspension
into sealed yogurt packages which contained headspace compositions
sufficient to induce a morphological switch as described in
Section 2.2. These conditions most closely mimicked actual product
conditions and allowed for the quantification of headspace compositional
changes in the challenge study. For challenge studies, inoculum
was added to skim milk containing lactic acid bacteria (LAB). This was
then transferred to retortable pouches (Amcor Limited, Hawthorne,
Victoria, Australia) and heat-sealed. Bags were incubated at 42 °C for