Fig. 3 – Effect of luteolin and luteolin glycosides on the LPS-induction of NO production. Cells were maintained in culture medium (Ctr) or pre-incubated with either 50 or 100 M luteolin (Lut), luteolin 7-O-β-glucopyranoside
(L7OG), isoorientin (IsoO), cassiaoccidentalin B (CasB) or orientin (O) for 1 h before stimulation with LPS for 24 h. Nitrite levels in the culture supernatants were evaluated by the Griess reaction and results expressed relatively to LPS-treated cells. Each value represents mean ± SEM of at least 3 independent experiments (###p < 0.001, when compared to control; **p < 0.01 and ***p < 0.001, when compared to
LPS).