Muscle all-trans-βC and 9-cis-βC concentrations in the present study increased linearly with increasing βC supplementation. However, muscle retinol, RP, and RA concentrations were not affected by increasing βC supplementation, suggesting that a large portion of supplement was stored in intramuscular fat as βC rather than RP. Conversely, Pickworth, Loerch, and Fluharty (2012b) demonstrated an increase in muscle retinol concentrations in steers supplemented with RP (5010 IU/kg) compared to steers not supplemented with RP (1260 IU/kg). Our data indicates that the extra dietary βC is stored as βC in tissue andmay bemetabolized to RA rather than being stored as RP.