FIG. 3. Identification of Leuconostoc species. PCR-amplified
DNAs of regions Vl (lane 2 to 4) and V3 (lane 5 to 7) of 16S rRNA
genes obtained by using primers P1 + P2 and P3 + P4, respectively,
were separated on a 2% agarose gel (A) and stained with ethidium
bromide. Lane 1, pUC18 digested with HpaII (19); lanes 2 and 5, L.
mesenteroides NCFB 523; lanes 3 and 6, L. lactis NCFB 533; lanes
4 and 7, L. paramesenteroides NCFB 503. Gels run in parallel,
which contained identical samples, were blotted and hybridized with
the PLC (B), the PLCI (C), or the PLCm (D) probe.