Determination of flavonoid compound
The AlCl3 method was used for determination of the total flavonoid content of the sample extracts according to the method described by Jagadish et al. (2009) with some modifications. An aqueous or ethanol extracts (1.5 ml) was mixed with 5 ml of deionized distilled water (ddH2O) and 0.3 ml of 5% NaNO2. After five min incubation at room temperature, 1.5 ml of 2% Aluminium trichloride (AlCl3) solution was added. Two milliliters of 1M NaOH was added after the next 6 min. The mixture was vigorously shaken on orbital shaker for 5 min at 200 rpm and the absorbance was read at 510 nm against a blank. Quercetin with different concentrations was used as a standard.