For histopathology, the kidney
tissues were immediately transferred to10 % formal saline
for paraffin embedding and staining with Hematoxylin &
Eosin (H&E), Periodic Acid-Schiff (PAS) (Malatiali et al.,
2008) and Masson’s trichrome and Gomori’s methanamine
silver nitrate (G.M.S.) stains. For enzyme estimation, kidney
tissues were immediately excised, washed in ice-cold saline,
blotted on tissue paper and immediately transferred to
phosphate buffer (pH 7.4) for preservation.