nother advantage with the recombinant P450 1A2 and NADPH-P450 reductase expression system is that the reactive metabolites will be generated inside the cell rather than outside the cell, as is seen with the S9 rat liver mix. As a result, this mode of bioactivation will allow short half-life reactive mutagens enough time to reach DNA sites allowing for an increase in sensitivity to mutagenesis.
The recombinant P450 protein also serves as a cost effective and animal friendly alternative to the S9 rat liver extract mix which is the current bioactivation standard.