The plates with dried surfaces were cultured at 28°C, irradiated under black fluorescent lamps (National: FL20S BL-B) for 15 days, and then the conidia on the surface were washed with sterilized water and adjusted to 105 conidia/mL. Young, healthy, and fully expanded green leaves of cassava cultivar SC8, which were cultivated for two months, were surface-sterilized, punched with sterile needles, inoculated with 20 μL conidia solution, and kept under humid conditions for 36 h. Sterile water was used as a control. Leaves were observed every day until lesions formed.