The present study demonstrates the production and properties of a biosurfactant isolated from marine
Streptomyces species B3. The production of the biosurfactant was found to be higher in medium containing
sucrose and lower in the medium containing glycerol. Yeast extract was the best nitrogen source for
the production of the biosurfactant. The isolated biosurfactant reduced the surface tension of water to
29 mN/m. The purified biosurfactant was shown critical micelle concentrations of 110 mg/l. The emulsifying
activity and stability of the biosurfactant was investigated at different salinities, pH, and temperature.
The biosurfactant was effective at very low concentrations over a wide range of temperature, pH,
and salt concentration. The purified biosurfactant was shown strong antimicrobial activity. The biosurfactant
was produced from the marine Streptomyces sp. using non-hydrocarbon substrates such as sucrose
that was readily available and not required extensive purification procedure. Streptomyces species B3 can
be used for microbially enhanced oil recovery process.