Trehalose can protect organisms against disadvantage conditions, such as high osmolarity, heat, oxidation, desiccation and freezing. the results showed that the ability to produce trehalose of soy sauce yeasts. The original strains is Zygosaccharomyces rouxii (S) and Torulopsis versatilis (T). The mutant strains is S3-2 and T3-5 were obtained by genome rearrangement on the basis of the original ones. The mutant strains have more advantages than the original strains in the tolerance of salt. The culture media was corn juice 13 Brix. The soy sauce yeast strains could accumulate more trehalose under salt and heat shock. The results showed that trehalose could be accumulated gradually in logarithmic phase and achieve a concentration peak in stationary phase. Then the relevance of growth conditions and intracellular trehalose level of soy sauce yeasts was investigated. Mutant strains which can grow in hypertonic conditions. But the optimal salt stimulate concentration was different. the wild yeast strains could accumulate most in corn juice in 9% salt, while the mutants in 12% salt. The original strains of the trehalose level reached the peak at 40°c temperature for 30 mins. The mutant yeast strains could accumulate less trehalose than the original.