Plate count of the selected bacteria
(Escherichia coli, Salmonella typhimurium, Shigella
dysenteriae, Pseudomonas aeruginosa) considerably
declined (90–99 %) in the biofilm after seventh and
fifth weeks of incubation under aerobic and anaerobic
conditions, respectively, indicating a positive shift
from pathogenic to beneficial microbial community.
While most probable number index of fecal coliforms
and E. coli in the sludge showed more reduction (98
and 99 %) under aerobic as compare to anaerobic
conditions (86 and 91 %) after 9 weeks of biofilm
formation on PS cubes.