. As Spirogyra
cell wall is purely cellulosic and cell contains simple starch. In fact pretreatment will damage the cellulose
leading to less yield of alcohol when compared with untreated spirogyra biomass. Also the results obtained from
the method of stationary method showed highest yield of free sugar released and alcohol produced than the
shaking method. This also helps in elimination of shaking method and thereby cutting the cost of power
consumption.