3.4 Cytotoxicity assay
The cytotoxicity assay employed the colorimetric
method [21]. Ellipticine, the reference
substance, exhibited activity toward
KB, BC, and NCI-H187 cell lines, with the
IC50 range of 0.3–0.6mg/ml (Table 2).
Acknowledgements
We are grateful to the Thailand Research Fund,
the Commission on Higher Education (Grant
No. MRG5280148), and Prince of Songkla
University for the financial support. We would
like to extend our appreciation to Assistant
Professor Dr Surat Laphookhieo, School of
Science, Mae Fah Luang University for giving
valuable suggestions and to Dr Sergey Novikov,
Faculty of Science and Industrial Technology,
Prince of Songkla University for
English editing.