ABSTRACT: High-pressure liquid chromatography (HPLC) has established itself as a
critically important analytical method for many research and commercial laboratories.
Employers expect today’s chemistry graduates to have a working knowledge of liquid
chromatography techniques including HPLC. In addition to HPLC, it is becoming more
important to educate students about newer separation technologies such as ultra-highpressure
liquid chromatography (UHPLC). Unfortunately, these systems cost
significantly more than traditional HPLC instruments, preventing their broad utilization
in instrumental teaching laboratories. We have developed an ultra-fast isocratic
separation method using traditional HPLC instrumentation that separates five
compounds in 1 min. When utilized as a teaching activity, this ultra-fast separation
allows students to develop an analytical method, generate standard calibration curves,
and analyze unknown samples in a single teaching laboratory period. Furthermore,
chromatograms obtained with this new method are similar to UHPLC, allowing
students to experience separations similar to those obtained on newer UHPLC systems
using a traditional HPLC.
ABSTRACT: High-pressure liquid chromatography (HPLC) has established itself as a
critically important analytical method for many research and commercial laboratories.
Employers expect today’s chemistry graduates to have a working knowledge of liquid
chromatography techniques including HPLC. In addition to HPLC, it is becoming more
important to educate students about newer separation technologies such as ultra-highpressure
liquid chromatography (UHPLC). Unfortunately, these systems cost
significantly more than traditional HPLC instruments, preventing their broad utilization
in instrumental teaching laboratories. We have developed an ultra-fast isocratic
separation method using traditional HPLC instrumentation that separates five
compounds in 1 min. When utilized as a teaching activity, this ultra-fast separation
allows students to develop an analytical method, generate standard calibration curves,
and analyze unknown samples in a single teaching laboratory period. Furthermore,
chromatograms obtained with this new method are similar to UHPLC, allowing
students to experience separations similar to those obtained on newer UHPLC systems
using a traditional HPLC.
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