Fresh Capsicum fruit pulp and placenta were separated and ground with
mortar and pestle. One gram of each part was placed into a round bottom flask and added with 25 ml
acetone. The sample was refluxed at 62°C for 1 h and the residual was removed by filtering through a
Whatman® filter paper No.1. For making up the volume of filtering solution into 25 ml, acetone
solution was added. The extract solution was then centrifuged at 9000 rpm for 10 min and the
supernatant was used for capsaicin purification.