The E. faecium BDU7 isolated from Ngari showed acid and bile tolerance establishing a possibility of the isolate to be considered as a probiotic strain. EPS are important extracellular bioactive molecules with potential biological and therapeutic activities. The present study evidenced that EPS from E. faecium BDU7 showed strong DPPH and superoxide radical scavenging ability in vitro. Considering its potency as a potential antioxidantthe extracted EPS can find wide application in functional food and pharmaceutical formulations.