Method validation
Linearity: The calibration curves of rhein and aloeemodin
reference standards were made from 5
concentrations. The concentrations of rhein and aloeMethod validation
Linearity: The calibration curves of rhein and aloeemodin
reference standards were made from 5
concentrations. The concentrations of rhein and aloeemodin
were ranged from 1.92-9.60 μg/ml and 1.56-
25.00 μg/ml, respectively. These solutions were added
with 0.5% w/v magnesium acetate in methanol and
adjusted to 25 ml volume with methanol. Maximum
absorbance of each mixture was measured at 515 nm
by a UV-vis spectrophotometer. Each sample was done
in triplicate. The relation between concentrations and
absorbance was plotted. The linearity was evaluated by
regression analysis and residual sum of squares.