The probe for the detection of the genus Lactobacillus is labeled with the 5
reporter dye VIC and the 3 quencher NFQ-MGB (Applied Biosystems, Nieuwerkerk
a/d IJssel, The Netherlands). The different lactobacilli species probes
are labeled with the 5 reporter dye 6-carboxy-fluorescein (FAM) and the 3
quencher NFQ-MGB (Applied Biosystems, Nieuwerkerk a/d IJssel, The Netherlands).
To even further increase specificity and sensitivity, TaqMan minor
groove binding probes were used (22).