Shallot extract was active against microbes at pH 4-8. Relative activities of shallot extract at temperature –7 to 121ฐC were 88 to 100 %. Extract of shallot only was soluble in dimethyl sulphoxide, dimethyl formamide and water. The enzymes and detergents used in this study had no effect on antimicrobial activity on water extract of shallot. Relative antimicrobial activity at incubation times of one hour to6 mounts were 94 to 100 %.